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KMID : 1098419970050030196
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Korean Journal of Medicinal Crop Science
1997 Volume.5 No. 3 p.196 ~ p.201
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Characterization of Cultured Angelica gigas Microspores by Flow Cytometry
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Park Chung-Heon
Seong Nak-Sul
Yu Hong-Seob
Peter Pauls K.
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Abstract
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To characterize active cells during microspore culture of Angelica gigas, flow cytometric and epifluorescent techniques were applied. The knowledge obtained from these types of studies will give us insight into early stage in plant development and may lead to the application of microspore-derived from haploid plants for breeding in recalcitrant species. Viability of cultured microspore differed depending on the developmental stages. Frequencies of active cells from tetrad, uni-nucleate, bi-nucleate and matured pollen were 12.8, 49.3, 42.3 and 31.7%, respectively. Alive microspores have luminescent the green fluorescence stained with FDA and blue fluorescence stained with DAPI.
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KEYWORD
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Micorspore culture, Angelica gigas, Flow Cytometry
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